A method of preparing a sterilized heart valve, the method comprising: compressing a compressible frame of a heart valve from an expanded configuration to a crimped configuration； the heart valve comprising the frame and a plurality of leaflets coupled to the frame； wherein each of the plurality of leaflets comprises a dry, unfixed, decellularized, antigen-free biological tissue that has been treated with a solution comprising a polyol or polyhydric alcohol； packaging the heart valve within a sealed packaging system while the heart valve is in the crimped configuration； and sterilizing the heart valve packaged within the sealed packaging system with one or more cycles of electron beam radiation.
