The present invention relatives to a strain ofSaccharomyces cerevisiae550C1 which is able to produce xylitol under the condition of without adding glucose. The present invention relatives to a method for producing xylitol by the strain ofSaccharomyces cerevisiae 550C1 which comprises hydrolyzing a lignocellulosic material to obtain a cellulose hydrolysate containing xylose and fermenting the cellulose hydrolysate to transform xylose to xylitol by the strain ofSaccharomyces cerevisiae550C1. By the strain ofSaccharomyces cerevisiae550C1 and the method for producing xylitol, the strain ofSaccharomyces cerevisiae550C1 is able to produce xylitol under the condition of without adding glucose.本發明係提供一種釀酒酵母菌(Saccharomyces cerevisiae550C1)，該釀酒酵母菌可在不加入葡萄糖饋料的條件下生產木糖醇。本發明同時提供一種生產木糖醇之方法，該方法使用上述釀酒酵母菌，該方法包含下列步驟：(a) 將木質纖維原料進行水解取得纖維素水解液，該纖維素水解液中含有木糖；及(b) 以如上所述之釀酒酵母菌發酵該纖維素水解液，將木糖轉化木糖醇。藉由如上所述之釀酒酵母菌及以其生產木糖醇之方法，該釀酒酵母菌可在不加入葡萄糖饋料的條件下生產木糖醇。
