Самуйленко Анатолий Яковлевич (RU),Мельник Николай Васильевич (RU),Гринь Светлана Анатольевна (RU),Мельник Роман Николаевич (RU),Сусский Евгений Владимирович (RU),Дадасян Артур Яппарович (RU),Беленко
申请号：
RU2015147811
公开号：
RU2015147811A
申请日:
2015.11.09
申请国别(地区):
RU
年份:
2017
代理人:
摘要：
FIELD: biotechnology.SUBSTANCE: method involves hyperimmunization of producer-oxen with a formalin-inactivated antigen isolated from the Fusobacterium necrophorum "0-1" VIEV production strain. The antigen is obtained by cultivation of the Fusobacterium necrophorum "0-1" VIEV production strain. Further, the culture liquid is subjected to ultrafiltration with a pore size of 13-20 kDa, 8-10% of Fusobacterium necrophorum "0-1" VIEV strain bacterial mass is added to the ultrafiltrate to Fusobacterium necrophorum "0-1" VIEV content of 1.5-2.0 billion of cells in 1 cm3, sodium salt of succinate chitosan is added at a final weight concentration of 0.5-1.5%. Inactivation is perfomed using with formalin, taken at a final concentration of 0.3-0.4% for 12-14 days at 37-38°C. Then, the resulting antigen is sorbed on 10-15% aluminium hydroxide in a glycol buffer with pH of 8.4-8.6, taken at a final concentration of 1.8-2.0%. Hyperimmunization of producer-oxen is first carried out with a vaccine to prevent farm animals necrobacteriosis at a dose of 0.4-0.6 ml/animal, then 4-5 weeks later - with the antigen obtained by the method described above at the rate of 1.5-2.0 ml of antigen per 100 kg of animal body weight, at first once, intradermically to the pre-laryngeal inguinal lymph node, together with the oil adjuvant, taken at a weight ratio of 1:0.8-1.0 to the antigen. Then twice with an interval of 5-7 days 0.5-0.6 ml of antigen per 100 kg of animal body weight is injected intradermally, first to the right pre-lobular and left inguinal lymph nodes, then to the left progloidal and right inguinal lymph nodes.EFFECT: method allows to obtain high quality serum foranimals necrobacillosis prevention and treatment.4 ex
