偵測AAV之方法农业专利-农业学术服务平台

发明人:: JIN, XIAOYING,金　晓英,金　曉英,O'RIORDAN, CATHERINE,奥瑞尔登　凯瑟琳,奧瑞爾登　凱瑟琳,LIU, LIN,刘　琳,劉　琳,ZHANG, KATE,张　晓葵,張　曉葵,金晓英,金曉英,ORIORDAN, CATHERINE,奥瑞尔登凯瑟琳,奧瑞爾登凱瑟琳,刘琳,劉琳,张晓葵,張曉葵

摘要：: Provided herein are methods for determining the serotype of a virus particle and/or or determining the heterogeneity of a virus particle (e.g., an AAV particle). In other embodiments, the invention provides methods to determine the heterogeneity of AAV particles. In some aspects, the invention provides viral particles (e.g., rAAV particles) with improved stability and/or improved transduction efficiency by increasing the acetylation and/or deamidation of capsid proteins.本文中提供了用於測定病毒顆粒的血清型和/或測定病毒顆粒(例如AAV顆粒)的異質性的方法。在其它實施方案中，本發明提供了測定AAV顆粒的異質性的方法。在一些態樣，本發明藉由增加殼體蛋白的乙醯化和/或脫醯胺化來提供具有改善的穩定性和/或改善的轉導效率的病毒顆粒(例如，rAAV顆粒)。