Disclosed are a probe combination for HPV virus genotyping detection based on an EFIRM technique, a kit and a method, relating to the field of gene detection technology. The probe combination comprises a plurality of probe pairs, and each probe pair is directed towards one HPV virus genotype, can detect the genotype of the HPV virus in a sample to be detected, and has a relatively strong specificity, a relatively high sensitivity and a relatively low false positive rate. The detection results are accurate and reliable.
