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Determination of withaferin a in rat plasma by high performance thin layer chromatography(hptlc) method.
专利权人:
NANCY PANDITA SCHOOL OF SCIENCE SVKM S NMIMS DEEMED TO BE UNIVERSITY
发明人:
NANCY PANDITA,NANCY PANDITA,SWETA JHA,SWETA JHA
申请号:
IN687/MUM/2014
公开号:
IN2014MU00687A
申请日:
2014.02.26
申请国别(地区):
IN
年份:
2015
代理人:
摘要:
The present invention describes quantitative determination of withaferin A from Rat plasma by High Performance Thin Layer Chromatographic Method. HPTLC is most widely used at industrial level for routine analysis of herbal medicines. Moreover HPTLC is user friendly and cost effective. We have tried to use such technique for determination of plant constituent from biological sample i:e blood after oral administration. The object of the present invention is to provide a novel method for determination of withaferin A from Rat plasma by using HPTLC method. We found that the adopted high performance thin layer chromatographic (HPTLC) method is capable of evaluating withaferin A in plasma after extraction. The method could be used to carry out pharmacokinetic studies of the withering A.
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