This invention is directed to a multi-layered tissue graft comprising a collagen layer and at least one separated and washed placental tissue component and/or umbilical cord component, wherein the collagen is human collagen substantially free of non-human antigens.Cette invention concerne une greffe de tissu multicouche comprenant une couche de collagène et au moins un composant tissu placentaire séparé et lavé et/ou un composant cordon ombilical, le collagène étant du collagène humain sensiblement exempt dantigènes non humains.