The invention discloses an artificial oil body carrier used for targeted breast cancer therapy, and preparation method and application of the same. The pJO1-oleZH2 plasmid is transformed into the host cell Escherichia coli BL21 (DE3), the oleZH2 protein is produced by induction, and then the oil and the oleZH2 protein are mixed to construct an artificial oil body carrier. In vitro and in vivo experiments, the artificial oil body carrier disclosed by the invention is confirmed that it can be effectively applied to target therapy, and the oil body carrier coated with fluorescent substances can reflect HER2/neu positive cancer cells, hence specifically detects breast cancer cell with over expression of HER2/neu. The preparation process is simple and very low environmental pollution. The artificial oil body carrier is highly stable in blood cell or blood serum, and is economical, highly efficient, convenient for use, small in size and easy to transport. The artificial oil body carrier can be coated with different oil-soluble breast cancer prevention drugs, which can effectively kill breast cancer cells and specifically detect and perform targeted therapy to breast cancer cells after being coated with the drug.La présente invention concerne un support corporel artificiel dhuile utilisé pour la thérapie ciblée du cancer du sein et son procédé de préparation et dapplication. Le plasmide pJO1-oleZH2 est transformé en lEscherichia coli BL21 (DE3) de cellule hôte, la protéine oleZH2 est produite par induction, et ensuite lhuile et la protéine oleZH2 sont mélangées pour construire un support corporel artificiel dhuile. Des expériences in vitro et in vivo ont confirmé que le support corporel artificiel dhuile selon linvention peut être appliqué efficacement à la thérapie ciblée, et le support corporel dhuile enrobé de substances fluorescentes peut entraîner la réflexion de cellules cancéreuses positives de HER2/neu, donc la détection spécifique de cellule cancéreuse du sein ave
