The process of simple production of recombinant vaccines for animals against toxins alpha, beta and epsilon of Clostridium perfringens and vaccines obtained
CARLOS EDUARDO POUEY DA CUNHA,CLÓVIS MOREIRA JUNIOR,FABRICIO ROCHEDO CONCEIÇÃO,FELIPE MASIERO SALVARANI,GUSTAVO MARÇAL SCHMIDT GARCIA MOREIRA,MARCELO MENDONÇA,MARCOS ROBERTO ALVES FERREIRA,PAULA FONSE
申请号：
BR102017011560
公开号：
BR102017011560A2
申请日:
2017.05.31
申请国别(地区):
BR
年份:
2018
代理人:
摘要：
The present invention relates to the production and use of bacterinas, cell lysates and purified antigens, obtained via the technology of recombinant DNA.As vaccines or vaccine formulations against alpha toxin components (CPA), beta (CPB) and epsilon (ETX) of Clostridium perfringens in animals.Synthetic genes coding for toxins and native CPA, CPB, ETX were cloned in vector expression and used to transform Escherichia coli strains.After the expression of recombinant antigens, the cultures were inactivated (vaccine 1), lysed (vaccine) or processed to obtain the recombinant antigens (purified vaccine (3), which.Separately, were adsorbed on aluminium hydroxide and used as a vaccine for the prevention of diseases caused by toxins, CPA, CPB, ETX of C. perfrigens.The vaccine formulations induced neutralising antibody titres above the recommended by the Ministry of agriculture, livestock and supply (mapa).Therefore,The vaccines produced by the technology of recombinant DNA used in the form not purified decreases the cost of production and therefore are a viable alternative for use in medicine goVeterinary.a presente invenção refere-se à produção e uso de bacterinas, lisados celulares e antígenos purificados, obtida via tecnologia do dna recombinante, como vacinas ou componentes de formulações vacinais contra toxinas alfa (cpa), beta (cpb) e épsilon (etx)de clostridium perfringens em animais. genes sintéticos e nativos que codificam para as toxinas cpa, cpb e etx foram clonados em vetor de expressão e utilizados para transformar cepas de escherichia coli. após a expressão dos antígenos recombinantes, as culturas foram inativadas (vacina 1), lisadas (vacina 2) ou processadas para obtenção dos antígenos recombinantes purificados (vacina 3), os quais, separadamente, foram adsorvidos em hidróxido de alumínio e usados como vacina para a prevenção de enfermidades causadas pelas toxinas cpa, cpb e etx de c. perfrigens. as formulações vacinais induziram títulos de anticorpos neutral
