SABBADINI, ROGER,GARLAND, WILLIAM,HANSEN, GENEVIEVE,JONES, STEVEN,WILLIAMS, DAVID
申请号：
ES07871262
公开号：
ES2524059T3
申请日:
2007.10.26
申请国别(地区):
ES
年份:
2014
代理人:
摘要：
An isolated antibody or antibody fragment that binds sphingosine-1-phosphate (S1P) under physiological conditions, in which: a. The antibody comprises two light chain polypeptides and two heavy chain polypeptides, in which: (i) each light chain polypeptide comprises the first, second, and third regions that determine complementarity CDRL1, CDRL2, and CDRL3, respectively, in that CDRL1 comprises the amino acid sequence ITTTDIDDDMN (SEQ ID NO: 10)； CDRL2 comprises the amino acid sequence EGNILRP (SEQ ID NO: 11)； and CDRL3 comprises the amino acid sequence LQSDNLPFT (SEQ ID NO: 12)； and (ii) each heavy chain polypeptide comprises the first, second, and third regions that determine the complementarity CDRH1, CDRH2, and CDRH3, respectively, wherein CDRH1 comprises the amino acid sequence DHTIH (SEQ ID NO: 13)； CDRH2 comprises the amino acid sequence AISPRHDITKYNEMFRG (SEQ ID NO: 31)； and CDRH3 comprises the amino acid sequence GGFYGSTIWFDF (SEQ ID NO: 15)； and b. the antibody fragment has a single antigen binding site and comprises a light chain polypeptide or fragment thereof and a heavy chain polypeptide or fragment thereof, in which: (i) the light chain polypeptide or fragment thereof it comprises the first, second, and third regions that determine the complementarity CDRL1, CDRL2, and CDRL3, respectively, in which CDRL1 comprises the amino acid sequence ITTTDIDDDMN (SEQ ID NO: 10)； CDRL2 comprises the amino acid sequence EGNILRP (SEQ ID NO: 11)； and CDRL3 comprises the amino acid sequence LQSDNLPFT (SEQ ID NO: 12)； and (ii) the heavy chain polypeptide or fragment thereof comprises the first, second, and third regions that determine the complementarity CDRH1, CDRH2, and CDRH3, respectively, in which CDRH1 comprises the amino acid sequence DHTIH (SEQ ID NO: 13)； CDRH2 comprises the amino acid sequence AISPRHDITKYNEMFRG (SEQ ID NO: 31)； and CDRH3 comprises the amino acid sequence GGFYGSTIWFDF (SEQ ID NO: 15), in which the three light chain CDRs are grafted into a human
