An ultrasound observation device includes: a processor configured to perform comparison operation on first scan data and second scan data during sequential alternating scan for generating Doppler information based on multiple sets of scan data acquired by sequentially transmitting ultrasound waves in multiple depth directions and by repeatedly transmitting the ultrasound waves in this sequential order, the first scan data being acquired by transmitting the ultrasound waves in a depth direction for first scan during the sequential alternating scan, the second scan data being acquired by transmitting the ultrasound waves for a second time during the sequential alternating scan and being acquired by transmitting the ultrasound waves in a depth direction identical to the depth direction for the first scan; and a sequential-alternating scan controller configured to control transmission timing for repeatedly transmitting the ultrasound waves during the sequential alternating scan based on a calculation result of the processor.