A uterine manipulator operable to detach the uterus and cervix from the vagina. The uterine manipulator includes a shaft with a first end and a second end, a handle coupled to the first end, and a tip assembly coupled to the second end and adapted for insertion into the uterus. The uterine manipulator also includes a cutting assembly coupled around the shaft and adapted to receive the cervix when the tip assembly is inserted into the uterus. The cutting assembly includes a cup portion and a cutter wire. The cup portion is configured to be positioned at a juncture between the cervix and the vagina and includes with two grooves around its circumference. The cutter wire is configured to be positioned between the two grooves when in a stored position, and then pulled from between the two grooves through tissue at the juncture to cut the tissue.