Barry Andrew;Provencher Laurel;Cohen Seth;Chen I-Jane;Yan Jun;Wang Jingjing
发明人:
Barry Andrew,Provencher Laurel,Cohen Seth,Chen I-Jane,Yan Jun,Wang Jingjing
申请号：
US201715830777
公开号：
US2018149647(A1)
申请日:
2017.12.04
申请国别(地区):
美国
年份:
2018
代理人:
摘要：
A microfluidic Western blot method and system including a microfluidic western blot method for immunoassay of proteins, the method including introducing a sample including the proteins onto a chip; electrophoretically separating the proteins; binding the separated proteins to beads to form protein-attached beads, the beads being magnetic; flowing the protein-attached beads into a magnetic holding region; applying a magnetic field to the magnetic holding region to fix the protein-attached beads in place within the magnetic holding region; binding primary antibodies to target proteins on the protein-attached beads; binding secondary antibodies to the bound primary antibodies; and detecting the bound secondary antibodies.
