COMPOSITIONS AND METHODS FOR RAPID IN VITRO SYNTHESIS OF BIOCONJUGATE VACCINES IN VITRO VIA PRODUCTION AND N-GLYCOSYLATION OF PROTEIN CARRIERS IN DETOXIFIED PROKARYOTIC CELL LYSATES
Michael Christopher Jewett,Jessica Carol Stark,Matthew P. DeLisa,Thapakorn Jaroentomeechai
申请号：
US16357820
公开号：
US20190284600A1
申请日:
2019.03.19
申请国别(地区):
US
年份:
2019
代理人:
摘要：
Disclosed are methods, systems, components, and compositions for cell-free synthesis of glycosylated carrier proteins. The glycosylated carrier proteins may be utilized in vaccines, including anti-bacterial vaccines. The glycosylated carrier proteins may include a bacterial polysaccharide conjugated to a carrier, which may be utilized to generate an immune response in an immunized host against the polysaccharide conjugated to the carrier. The glycosylated carrier proteins may be synthesized in cell-free glycoprotein synthesis (CFGpS) systems using prokaryote cell lysates that are enriched in components for glycoprotein synthesis such as oligosaccharyltransferases (OSTs) and lipid-linked oligosaccharides (LLOs) including OSTs and LLOs associated with synthesis of bacterial O antigens.
