Gong, Guochun,Lai, Ka-Man Venus,Valenzuela, David M.
申请号：
AU2013352235
公开号：
AU2013352235A1
申请日:
2013.11.26
申请国别(地区):
AU
年份:
2015
代理人:
摘要：
Genetically modified somatic cells of a non-human animal are provided that are engineered to contain a self-excisable, recombinase expression cassette comprising a site- specific recombinase gene operably linked to an ES cell-specific promoter. Compositions and methods for producing a genetically modified, cloned non-human animal that is free of a selective marker gene and a recombinase gene are provided, wherein a targeting construct comprising a self-excisable recombinase gene operably linked to an ES cell-specific promoter is introduced into differentiated somatic cells. The genetically modified genome of the somatic cells is transferred into an enucleated host oocyte. The artificially created zygote is then cultured
