A tamper-evident closure assembly includes a connector, a tamper element removably affixed to the connector, and a cap having an interior surface defining a receptacle, where a portion of the connector is within the receptacle and the cap is configured to engage with the tamper element. The tamper element is configured to be separated from the connector upon application of a removal force and to be retained within the receptacle of the cap so that it can later be removed from the receptacle. A kit for a tamper-evident closure assembly includes a cap having an interior surface defining a receptacle, a tamper element configured to engage with the cap within the receptacle, and a housing configured to receive the cap.