[Problem] Provided is an anti-human Tie2 antibody for preventing or treating diabetic macular edema, diabetic retinopathy, and critical limb ischemia by binding to a human Tie2 to activate the human Tie2.[Means for Solution] The present inventors have conducted investigations on an anti-human Tie2 antibody, and have thus provided an anti-human Tie2 antibody comprising four heavy chain variable regions and four light chain variable regions, in which the heavy chain variable region consists of the amino acid sequence of the amino acid numbers 1 to 122 of SEQ ID NO: 2, the light chain variable region consists of the amino acid sequence of the amino acid numbers 1 to 113 of SEQ ID NO: 4, the one heavy chain variable region and the one light chain variable region constitute one antigen-binding site, and the antibody comprises four antigen-binding sites.提供藉由結合於人類Tie2,使人類Tie2活性化而預防或治療糖尿病黃斑部水腫、糖尿病網膜症或重症下肢缺血之抗人類Tie2抗體。本發明者等人對於抗人類Tie2抗體進行探討,來提供一種抗人類Tie2抗體,其係包含4個重鏈可變區域及4個輕鏈可變區域之抗人類Tie2抗體,其中該重鏈可變區域係由序列編號2之胺基酸編號1至122的胺基酸序列所構成、該輕鏈可變區域係由序列編號4之胺基酸編號1至113的胺基酸序列所構成,1個該重鏈可變區域與1個該輕鏈可變區域構成1個抗原結合部位,該抗體包含4個抗原結合部位。
