METHODS AND COMPOSITIONS FOR EXPANSION OF HEMATOPOIETIC STEM AND/OR PROGENITOR CELLS EMPLOYING A CYTOCHROME P450 1B1 (CYP1B1) INHIBITOR OR A MUSASHI-2 (MSI2) ACTIVATOR
A method of increasing the self-renewal and/or expansion of hematopoietic stem and/or progenitor cells (HSPCs) is described. Inhibiting the activity and/or expression of cytochrome P450 1B1 (CYP1B1) and/or increasing the expression or activity of Musashi-2 (MSI2) increases the expansion of HSPCs. The HSPCs may be cultured in the presence of a CYP1B1 inhibitor and/or a MSI2 activator. Optionally, the cells may be expanded ex vivo and transplanted into a subject in need thereof.
