WONG, BING LOU,黄炳鏐,黃炳鏐,WAI, NORMAN FUNG MAN,衞凤文,衞鳳文,KWOK, SUI YI,郭瑞仪,郭瑞儀,LEUNG, YUN CHUNG,梁润松,梁潤松
申请号：
TW103107618
公开号：
TWI634212B
申请日:
2014.03.06
申请国别(地区):
TW
年份:
2018
代理人:
摘要：
The present invention provides a pharmaceutical composition containing albumin-binding arginine deiminase fusion protein (AAD) for treating cancer or other arginine-dependent diseases. The AAD fusion protein can be purified from both soluble and insoluble fractions of crude proteins, it binds to human serum albumin (HSA) and has its high activity with longer half life for efficient depletion of arginine in cancer cells. The specific activities of wild-type ADI and AAD in the present invention are 8.4 and 9.2 U/mg (at physiological pH 7.4), respectively. The AAD used in the present invention can be used in the treatment of various cancers (e.g. pancreatic cancer, leukemia, head and neck cancer, colorectal cancer, lung cancer, breast cancer, liver cancer, nasopharyngeal cancer, esophageal cancer, prostate cancer, stomach cancer & brain cancer) and curing arginine-dependent diseases. The composition can be used alone or in combination with at least one chemotherapeutic agent to give a synergistic effect on cancer treatment and/or inhibiting metastasis.本發明提供一種含有白蛋白結合精胺酸去亞胺酶融合蛋白(AAD)之醫藥組成物,其用於治療癌症或其他精胺酸依賴性疾病。該AAD融合蛋白可自粗蛋白之可溶與不可溶部分純化,其結合於人類血清白蛋白(HSA)且其高活性及較長半衰期可有效消耗癌細胞中之精胺酸。本發明中之野生型ADI及AAD的特定活性分別為8.4及9.2U/mg(在生理學pH 7.4下)。本發明中所用之AAD可用於治療多種癌症(例如胰臟癌、白血病、頭頸部癌、結腸直腸癌、肺癌、乳癌、肝癌、鼻咽癌、食道癌、前列腺癌、胃癌及腦癌)並治癒精胺酸依賴性疾病。該組成物可單獨使用或與至少一種化學治療劑組合使用以對癌症治療及/或抑制轉移產生協同效應。
