The present invention relates to a recombinant vaccinia virus comprising a gene encoding sPD-1 or hyaluronidase and to a pharmaceutical composition comprising the same. The recombinant vaccinia virus comprising a cancer therapy gene and having suppressed expression of K3L, TK, or VGF gene of the vaccinia virus according to the present invention has an excellent anti-tumor effect. Therefore, the recombinant vaccinia virus of the present invention can be advantageously used in cancer treatment.
