The present invention provides a grass protection mat which can be used for protection of grass in a golf course, a grass field, and a park lawn, or be used for growth of the grass. The grass protection mat includes a body member constituting grass penetrating spaces, protruding pillars formed at upper portion of the body member, lug members formed at lower portion of the body member, and an outer frame provided at outer wall of the body member. The grass protection mat further includes sinking prevention means provided across the grass penetrating spaces formed at the lower portion of the mat, elastically deformable sinking prevention means, mat sliding prevention means provided to the sinking prevention means, and sliding prevention means. The invention may obtain improve effects that the grass protection mat prevents the mat from sinking into a ground after construction of the mat on the ground, enhances sinking prevention effect by elastically deforming the sinking prevention means, while preventing the mat sinking, restrains the mat from slipping through the mat sliding prevention means provided to the sinking prevention means under or after construction of the mat, and effectively prevents from sliding on the mat during passage of the mat.