PROBLEM TO BE SOLVED: To provide a method for promoting carotenoid production of lactic acid bacteria and for giving lactic acid bacteria resistance to oxidation stress without recombination technology.SOLUTION: The invention provides a method for producing carotenoid, that comprises aerobic culture of a lactic acid bacterium having carotenoid biosynthesis genes including crtN gene and crtM gene. The invention also provides a method for producing a lactic acid bacterium having improved carotenoid productivity and resistance to oxidation stress, as well as anti-oxidation composition comprising the lactic acid bacterium. The anti-oxidation composition may be food and drink or medicine.COPYRIGHT: (C)2015,JPO&INPIT【課題】組み換え技術なしに乳酸菌のカロテノイド生産を促進するともに、乳酸菌に酸化ストレス耐性を付与する方法を提供する。【解決手段】crtN遺伝子とcrtM遺伝子を含むカロテノイド生合成遺伝子を有する乳酸菌を好気培養する、カロテノイドの生産方法、および、カロテノイド生産性と酸化ストレス耐性が向上した乳酸菌の製造方法。前記乳酸菌を含む抗酸化性組成物。飲食品または医薬品である、前記抗酸化性組成物。【選択図】なし