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Plant transformation with in vivo assembly of a sequence of interest using a site-specific recombinase
专利权人:
Anatoly Giritch
发明人:
Anatoly Giritch,Serik Eliby,Sylvestre Marillonnet,Victor Klimyuk,Yuri Gleba
申请号:
US10544135
公开号:
US09228192B2
申请日:
2004.01.30
申请国别(地区):
US
年份:
2016
代理人:
摘要:
A process of producing transgenic plants or plant cells stably transformed on a chromosome with a DNA sequence of interest capable of expressing a function of interest, said process comprising (a) providing plant cells or plants with at least two different vectors that are adapted to recombine with each other between site-specific recombination sites compatible with a site-specific recombinase that is also provided in order to produce a non-replicating recombination product containing said DNA sequence of interest, (ii) said at least two different vectors are adapted for integrating said DNA sequence of interest into said chromosome, (iii) said DNA sequence of interest contains sequence portions from at least two of said at least two different vectors, said sequence portions being necessary for expressing said function of interest from said DNA sequence of interest and (b) selecting plants or plant cells expressing said function of interest.
来源网站:
中国工程科技知识中心
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