#$%^&*AU2020101602A420200917.pdf#####ABSTRACT The invention relates to a method for rapidly preparing stevia leaf protoplasts. Choosing tissue culture of young leaves of stevia as material. The most suitable conditions for isolation of leaf protoplasts of stevia were screened by controlling the factors such as the time of plasmolysis, the ratio and concentration of Onozuka R-10, hemicellulase, macrozyme R-10, and the concentration of mannitol in the enzymolysis solution, the pH value of the enzymatic hydrolysis solution, and the time of enzymatic hydrolysis. The method can obtain more free protoplasts and has high activity, which is a simple and quick method for preparing stevia leaf protoplasts.-1/3Figure 1
