EHBENS ALLEN DZH. ML.,ЭБЕНС Аллен Дж. мл.,GREHJ ALAN M.,ГРЭЙ Алан М.,LJAN VEHJ-CHIN,ЛЯН Вэй-Чин,U JAN,У Янь,JUJ SHAN-FAN,ЮЙ Шан-Фань
申请号：
RU2008152431/10
公开号：
RU0002436796C9
申请日:
2007.05.29
申请国别(地区):
RU
年份:
2013
代理人:
摘要：
FIELD: medicine.SUBSTANCE: there are offered versions of antibodies specific to CD22 epitope located from amino acid 22 to amino acid 240 CD22. There are disclosed: a coding polynucleotide, an expression vector, a based host cell and a method of producing an antibody with the use of the cell. There are described versions of a method of CD22 detection on the basis of the antibodies. There are disclosed versions of the CD22 immunoconjugate and based pharmaceutical compositions for treating disturbed B-cell proliferation, and also versions of a method of treating with the use of the pharmaceutical composition. There is disclosed a method of B-cell proliferation inhibition on a basis the immunoconjugate. There are described versions of an engineered cystein-substituted antibody specific to CD22 with one or more free cysteines of thiol reactance within the range 0.6 to 1.0. There are disclosed versions of the "antibody-drug" conjugate, the immunoconjugate and pharmaceutical formulaitons for treating disturbed B-cell proliferation. There are also described a method for protein CD22 detection in a sample on the basis of the immunoconjugate, a method for B-cell detection and a method of treating a malignant tumour on the basis of the "antibody-drug" conjugate. There are disclosed: a product for treating disturbed B-cell proliferation on the basis of the pharmaceutical formulation and a method of producing the "antibody-drug" conjugate.EFFECT: use of the invention provides new specific CD22 antibodies and the based drugs of acceptable therapeutic efficacy with lower toxicity that can find application in therapy of tumours.227 cl, 25 dwg, 16 tbl, 14 exНастоящее изобретение относится к иммунологии и биотехнологии. Предложены варианты антител, специфичных к эпитопу CD22, расположенному от аминокислоты 22 до аминокислоты 240 CD22. Раскрыты: кодирующий полинуклеотид, вектор экспрессии, клетка-хозяин на его основе и способ получения антитела с использованием клетки. Описаны вариан
