The present disclosure provides a bone age assessment and height prediction system including an image capturing unit and a non-transitory machine readable medium. The image capturing unit is for obtaining a target x-ray image data of a subject. The non-transitory machine-readable medium is for storing a program for assessing the development of the bones of a hand and the bone age of the subject, and predicting the adult height of the subject when executed by a processing unit. Therefore, the bone age assessment and height prediction system of the present disclosure can effectively improve the accuracy and sensitivity of the bone age assessment and the height prediction, and the time for assessing the bone age and predicting the height can be further shorten.本發明提供一種骨齡評估與身高預測系統,包含一影像擷取單元以及一非暫態機器可讀媒體。影像擷取單元用以取得一受試者的一目標手骨X光影像資料。非暫態機器可讀媒體用以儲存一程式,當程式由處理單元執行時用以判斷受試者之手骨發育狀態、骨齡和預測受試者之成年身高。藉此,本發明之骨齡評估與身高預測系統可有效提升骨齡評估及身高預測的準確度與敏感度,並可縮短骨齡評估及身高預測的判定時間。