PROBLEM TO BE SOLVED: To provide an ozone disinfection apparatus capable of conducting disinfection treatment for a short period of time, and to provide a disinfection method of the same.SOLUTION: In an endoscope disinfection apparatus 100 which disinfects an endoscope 10 that is arranged in a disinfection tank 2 and an object to be disinfected, by using ozone water, and discharges drainage water through a drain pipe 70, the endoscope disinfection apparatus 100 has an ozonolysis device 73 provided at the drain pipe 70, and discharges the ozone water after the disinfection treatment through the ozonolysis device 73. In addition, the endoscope disinfection apparatus 100 determines ozonolysis efficiency of the ozonolysis device 73 from a discharge period of time of the ozone water.