PROBLEM TO BE SOLVED: To provide an effective preventive means against Edwardsiella disease in Sparidae or Carangidae fishes.SOLUTION: The live vaccine formulation contains a live virus of a typical strain of Edwardsiella trade (e.g., HET-1 strain) which shows strong pathogenicity to fishes except Sparidae or Carangidae fishes, but has low pathogenicity or non-pathogenicity to Sparidae or Carangidae fishes, such as Anguilla japonica and Paralichthys olivaceus, and has strong immunogenicity against Edwardsiella disease in Sparidae or Carangidae fishes.