The present invention relates to a method for sorting highly effective mesenchymal stem cells for treating an immune disorder, the method comprising a step of measuring the level of an immunosuppressive biomarker following IFN-γ pretreatment simulation in mesenchymal stem cells； highly effective mesenchymal stem cells sorted by the method； and a method for treating an immune disorder by using the highly effective mesenchymal stem cells. The present invention can provide a useful method capable of obtaining functionally excellent mesenchymal stem cells having an immune reaction regulation capability and used for clinically treating various immune disorders including graft-versus-host disease and autoimmune disorders, and thus can be usefully used as a therapy for immune disorders.
