Gordon-Kamm, William J.,Lowe, Keith S.,Peterson, David J.,Scelonge, Christopher J.,St. Clair, Grace M.,Wang, Bing-Bing
申请号：
AU2010339481
公开号：
AU2010339481B2
申请日:
2010.12.30
申请国别(地区):
AU
年份:
2016
代理人:
摘要：
A variety of methods and compostions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism.
