THE ADMINISTRATORS OF THE TULANE EDUCATIONAL FUND;AXOSIM, INC.
发明人:
MOORE, MICHAEL JAMES,CURLEY, JAB LOWRY,SHARMA, ANUP D.,BOWSER, DEVON,BEHN, JORDAN
申请号：
CA3084259
公开号：
CA3084259A1
申请日:
2018.12.04
申请国别(地区):
CA
年份:
2019
代理人:
摘要：
The present disclosure generally relates to a cell culturing system, and specifically to a three-dimensional cell culturing system for neuronal cells that promotes both structural and functional characteristics that mimic those of in vivo peripheral fibers, including cell myelination. Using a dual hydrogel construct and spheroids comprising neuronal cells, the present disclosure provides methods, devices, and systems for in vitro spatially-controlled, three-dimensional models that permit intra- and extra-cellular electrophysiological measurements and recordings. The three-dimensional hydrogel constructs allow for flexibility in incorporated cell types, geometric fabrication, and electrical manipulation, providing viable systems for culture, perturbation, and testing of biomimetic neural growth with physiologically-relevant results.
