The present disclosure provides a method and system for mapping anatomical structures and marking them on an image to be printed on a substrate including the steps of inserting an imaging device into a surgical site, obtaining an image of a defect located in the surgical site from the imaging device, adjusting the image, transmitting the image to a printer, and printing the image on a substrate. The printed image may be a size directly proportional to the defect. The adjusting step may further include the steps of setting a minimum margin to be maintained between the perimeter of the defect and the perimeter of the substrate, selecting a shape and size of the substrate, and identifying at least one anatomical feature of the surgical site.
