Provided is a novel bacteriophage ΦCJ22 (KCCM11364P). In addition, provided is an antibacterial composition containing the bacteriophage ΦCJ22 (KCCM11364P) as an active ingredient. Further, provided is a method of preventing and/or treating infectious diseases caused by Clostridium perfringens in animals except for humans by using the bacteriophage ΦCJ22 (KCCM11364P) or an antibacterial composition containing the bacteriophage ΦCJ22 (KCCM11364P) as an active ingredient.