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Cell culture method
专利权人:
国立大学法人千葉大学
发明人:
山田 真澄,関 実,堀 綾香,平井 優,矢嶋 祐也,北川 陽一
申请号:
JP2016540188
公开号:
JP6628416B2
申请日:
2015.07.31
申请国别(地区):
JP
年份:
2020
代理人:
摘要:
Cells are embedded in a high density, contact with extracellular matrix components optionally cells, control of the three-dimensional form, tissue shape maintenance after the hydrogel is removed, efficient proliferation and cell-cell interactions cells in tissue formation and capable of uniform seeding of cells into tissues inside, provides a method for obtaining a fibrous protein material. Fibrous protein material forming method according to the invention comprises an inlet for introducing a first aqueous solution of sodium alginate and proteins are dissolved, an inlet for introducing the gelling agent solution, an inlet passage which is connected to the inlet, the inlet a merging flow path flow path are merged, to channel structure having an outlet located downstream of the confluent channel, continuously introducing a first aqueous solution and gelling agent solution in the flow channel structure, channel structure in the interior of the first aqueous solution is continuously gelled to form a hydrogel, the external or internal channel structure, the proteins contained in the hydrogel is chemically crosslinked, the alginic acid contained in the hydrogels Remove.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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