The invention is based on the discovery of the epitope in the Stx2 protein forthe 11 E1O antibody. The inventionfeatures compositions containing non-full length Stx2 polypeptides thatinclude the 11 E1O monoclonal antibody epitope. The inventionalso features methods of producing anti-Stx2 antibodies specific for the 11E1O epitope of the Stx2 protein. Additionally,the invention features methods for treating a subject having, or at risk ofdeveloping, a Shiga toxin associated disease (e.g.,hemolytic uremia syndrome and diseases associated with E. coli and S.dysenteriae infection) with a polypeptide that includes the11 E1O epitope or with an anti-Stx2 antibody developed using the methods ofthe invention. Furthermore, the invention featuresthe detection of Stx2 in a sample using the antibodies developed using themethods of the invention.
