具有抑制胃癌细胞BGC-823增殖功能的基因及其制备方法
- 专利权人:
- 上海大学
- 发明人:
- 陈媛媛,朱静嬿,李哲,武岳,余抗抗,宋一超,陈付学
- 申请号:
- CN201110431938.5
- 公开号:
- CN102653762A
- 申请日:
- 2011.12.21
- 申请国别(地区):
- CN
- 年份:
- 2012
- 代理人:
- 摘要:
- 本发明涉及一种具有抑制胃癌细胞BGC-823增殖功能的基因及其制备方法。该基因是根据胃癌细胞BGC-823设计出的Stat5b-shRNA的两条部分互补核苷酸序列,再将该两条互补核苷酸序列和市售SilenCircleTMRNAi转录试剂盒中的SilenCircle质粒进行连接,得到具有抑制胃癌细胞BGC-823增殖功能的基因。本发明设计对与胃癌细胞BGC-823增殖有关联的基因的RNAi,并应用胃癌细胞BGC-823模型研究其对胃癌细胞BGC-823增殖的抑制作用。为RNAi在胃癌治疗应用提供了一种新方法。
- 来源网站:
- 中国工程科技知识中心
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