A purified solution of human plasma, MNCs and platelets isolated from whole blood, bone marrow, or stromal vascular fraction cells separated from adipose tissue wherein said purified solution comprises at least 90% by number of the MNCs in said whole blood, bone marrow, or stromal vascular fraction cells separated from adipose tissue, said purified solution comprises at most 10% by number of the red blood cells and granulocytes in said whole blood, bone marrow, or stromal vascular fraction cells separated from adipose tissue, and said purified solution is isolated and separated from said whole blood, bone marrow, or stromal vascular fraction cells separated from adipose tissue during a single centrifugation run.