593926 Provided is a composition for stimulating an immune response against Stx2, comprising a pharmaceutically acceptable carrier and at least one purified polypeptide with a specified sequence, inserted into a non-Stx2 protein scaffold, wherein said polypeptide has the antigenicity of Stx2, wherein said scaffold comprises a protein substantially identical to Stx1, or a functional fragment thereof. Further provided are antibodies directed to the polypeptides and their use in diagnosing or treating Shiga toxin associated diseases.
