Juan Carlos IZPISUA BELMONTE,Keiichiro SUZUKI,Reyna HERNANDEZ-BENITEZ,Jun WU,Yuji TSUNEKAWA
申请号：
US16318120
公开号：
US20190225991A1
申请日:
2017.07.14
申请国别(地区):
US
年份:
2019
代理人:
摘要：
Disclosed herein are homology-independent targeted integration methods of integrating an exogenous DNA sequence into a genome of a non-dividing cell and compositions for such methods. Methods herein comprise contacting the non-dividing cell with a composition comprising a targeting construct comprising the exogenous DNA sequence and a targeting sequence, a complementary strand oligonucleotide homologous to the targeting sequence, and a nuclease, thereby altering the genome of the non-dividing cell.
