An undifferentiated stem cell removing agent containing at least one selected from the group consisting of fatty acid synthesis inhibitors, fatty acid utilization inhibitors, and cholesterol synthesis inhibitors. An undifferentiated stem cell removing method comprising culturing a cell mixture containing undifferentiated stem cells and differentiated cells in the presence of the undifferentiated stem cell removing agent. The manufacturing method of the cell for transplantation including the process of the following (i) and (ii). (I) a step of inducing desired differentiated cells from undifferentiated stem cells； and (ii) a step of culturing the cell mixture obtained by the step (i) in the presence of the agent for removing undifferentiated stem cells.脂肪酸合成阻害剤、脂肪酸利用阻害剤、及びコレステロール合成阻害剤からなる群から選ばれる少なくとも一種を含有する未分化幹細胞除去剤。未分化幹細胞及び分化細胞を含む細胞混合物を、前記未分化幹細胞除去剤の存在下で培養することを含む、未分化幹細胞除去方法。以下の（ｉ）及び（ｉｉ）の工程を含む、移植用細胞の製造方法。（ｉ）未分化幹細胞から所望の分化細胞を誘導する工程；及び（ｉｉ）前記工程（ｉ）により得られた細胞混合物を、前記未分化幹細胞除去剤の存在下で培養する工程
