MACDONALD LYNN,MURPHY ANDREW J,GURER CAGAN,MCWHIRTER JOHN
申请号：
IN201617031728
公开号：
IN201617031728A
申请日:
2016.09.17
申请国别(地区):
IN
年份:
2017
代理人:
摘要：
Genetically modified non human animals and methods and compositions for making and using them are provided wherein the genetic modification comprises (a) a deletion in an immunoglobulin constant region C1 gene (optionally a deletion in a hinge region) of a heavy chain constant region gene sequence and (b) replacement of one or all endogenous V D and J gene segments with at least one unrearranged light chain variable (V) gene segment and at least one unrearranged light chain joining (J) gene segment capable of recombining to form a rearranged light chain variable region (V/J) nucleotide sequence operably linked to the heavy chain constant region gene sequence comprising a deletion in the C1 gene and/or insertion of a genetically engineered single rearranged light chain wherein the mouse is capable of expressing a functional IgM single domain antigen binding proteins e.g. V single domain binding proteins and a genetically engineered rearranged light chain.
