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Automated generation of genetically modified stem cells
专利权人:
发明人:
Ian Johnston,Volker Huppert,Michael Essl,Stefan Miltenyi
申请号:
US14689105
公开号:
US09493789B2
申请日:
2015.04.17
申请国别(地区):
US
年份:
2016
代理人:
摘要:
The invention relates to a process for generation of genetically modified stem cells comprising the stepsa) providing a cell sample in suspension comprising stem cells in a centrifugation chamber comprising a base plate and cover plate connected by a cylinderb) adjusting the volumetric concentration of stem cells in the cell sample to at least 1×105 stem cells per mL cell sample by centrifugationc) introducing viral and/or non-viral vectors to the centrifugation chamber for genetically modifying the stem cellsd) adjusting the spatial concentration of stem cells in the centrifugation chamber by rotating the centrifugation chamber at a speed where the cell sample is located at the outermost 35% of the radius of the base plate of the centrifugation chamber, thereby inducing gene modification of the stem cells.
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