The present disclosure relates to a ligation device, an unlocking method, and a ligation instrument. The ligation device may include a clip and a limiting tube. A distal end of the clip may be used for ligation. The limiting tube may be provided with an accommodation channel, and a proximal end of the clip may be connected with the accommodation channel, and the proximal end of the clip may move into or out of the limiting tube. The clip may be provided with a locking portion, and the limiting tube may be provided with a locking position that is matched with the locking position. When the locking portion is locked with the locking position, the locking portion may be unlocked from the locking position by operating the locking portion. Alternatively, the clip may be provided with an unlocking portion that is coupled with the locking portion. The locking portion may be unlocked from the locking position by operating the unlocking portion. The ligation device may be re-opened after being locked for ligation, causing no secondary damage to the ligated tissue.
