PROBLEM TO BE SOLVED: To provide a sterilization method having excellent sterilization properties for such as germs in fluid such as cleaning fluid, or germs present in clothing.SOLUTION: A sterilization method includes a step of causing germs to have contact with a liquid composition obtained by mixing: (A) nonionic surfactant expressed by a general formula (1) below (B) quaternary ammonium compound expressed by a general formula (2) below and a liquid medium: RO-(EO)mH (1) [in the formula, R denotes a hydrocarbon group having 10-18 carbon atoms, EO denotes an ethyleneoxy group, and m denotes a number 1-12 in average addition mole number], and [in the formula, R1, R2 and R3 each independently denote an aliphatic hydrocarbon group having 6-8 carbon atoms, R4 denotes a hydrocarbon group having 6-8 carbon atoms, and X- denotes an anion.]COPYRIGHT: (C)2015,JPO&INPIT【課題】殺菌性、例えば洗浄液などの液体中の菌や衣料に存在する菌の殺菌性に優れた殺菌方法を提供する。【解決手段】(A)下記一般式(1)で表される非イオン界面活性剤、(B)下記一般式(2)で表される第4級アンモニウム化合物、及び液体媒体を配合して得られる液体組成物を、菌と接触させる工程を含む、殺菌方法。RO-(EO)mH (1)〔式中、Rは炭素数10以上、18以下の炭化水素基、EOはエチレンオキシ基、mは平均付加モル数で1以上、12以下の数である。〕【化1】〔式中、R1、R2及びR3はそれぞれ独立に炭素数6以上、8以下の脂肪族炭化水素基、R4は炭素数6以上、8以下の炭化水素基、X-は陰イオンである。〕【選択図】なし