Provided are triple helical bundle protein, polynucleotide coding the triple helical bundle protein, a preparation method of the triple helical bundle protein, and a pharmaceutical composition comprising the triple helical bundle protein as an effective component. The triple helical bundle protein includes at least one among the following amino acid substitutions of: a) at least one amino acid residue of a triple helical bundle protein corresponding to phenylalanine (F) which is a fifth amino acid of SEQ ID NO:2 or a third amino acid of SEC ID NO:3, with F b) at least one amino acid residue of a triple helical bundle protein corresponding to tryptophan (W) which is a ninth amino acid of SEQ ID NO:2 or a seventh amino acid of SEQ ID NO:3, with W and c) at least one amino acid residue of a triple helical bundle protein corresponding to leucin (L) which is a twelfth amino acid of SEQ ID NO:2 or a tenth amino acid of SEQ ID NO:3, with L.COPYRIGHT KIPO 20163중-나선 다발 단백질, 상기 3중-나선 다발 단백질을 암호화하는 폴리뉴클레오타이드, 상기 3중-나선 다발 단백질의 제조 방법, 및 상기 3중-나선 다발 단백질을 유효성분으로 포함하는 약학 조성물이 제공된다.
