The purpose of the present invention is to provide a hyaluronidase inhibitor that can be applied to a wide range of uses. The present invention is a hyaluronidase activity inhibitor containing [beta]-1,3-1,6-glucan in which a carboxymethyl group has been introduced to a portion of the hydroxy groups. This hyaluronidase activity inhibitor is particularly useful for cosmetic compositions.本發明之目的為提供可利用於廣泛用途之玻尿酸酶抑制劑。本發明係含有羥基的一部分經羧基甲基化之β-1,3-1,6-葡聚糖的玻尿酸酶活性抑制劑。該玻尿酸酶活性抑制劑係對化粧料組成物特別有益。