The application relates to the technical field of optical fiber sensing layer and provides an optical sensing fiber comprising two layers of pressure-sensitive assembly films which are vertically superposed and at least one layer of flexible and smooth film separating a space between the two layers of pressure-sensitive assembly film into at least two interlayer, each interlayer is provided with at least one optical fiber row which has many S-shaped concatenated cablings and extends along a first direction, the cablings of vertically adjacent optical fiber rows staggers and superposes. The optical fiber layer distributes the optical fiber rows uniformly on the pressure-sensitive assembly films to form optical fiber sensing areas, and the bending portions of the cablings of two vertically adjacent optical fiber rows stagger and superpose. The optical fiber sensing areas compensate their blind sensing areas mutually so that the characteristics that the optical fiber is brittle and can be changed by the optical fiber sensing layer are changed, and the breath, heartbeat, sleep, temperature and moisture can be detected quickly and precisely. The optical fiber sensing layer has the characteristics of a high and adjustable sensitivity, a high resolution, an electromagnetic interference resistance, and a uniform sensitivity. The application further provides a monitoring system comprising an optical fiber sensing layer, an illuminant, a light detecting assembly, an optical fiber transmission circuit and a processing circuit.