The present invention provides an extraction method of sturgeon collagen in low temperature , that is able to extract sturgeon collagen without through a heat process. In this way, it can make sturgeon collagen denature not easily, and further provides a product which is used in cosmetics or health care.本發明為提供一種低溫萃取鱘龍魚膠原蛋白的方法,其不需透過加溫的方式即能萃取鱘龍魚的膠原蛋白,此方法能使膠原蛋白不易變質,適用於化妝品美容及保健食品產業上。