An animal positioner capable of restraining movement of an animal without anesthesia is provided. The animal positioner includes two forelimb positioning members (6a, 6b) cylindrically attached to forelimbs (5a, 5b) of an animal (2), respectively, so as to wrap the forelimbs (5a, 5b); two hindlimb positioning members (8a, 8b) cylindrically attached to hindlimbs (7a, 7b) of the animal (2), respectively, so as to wrap the hindlimbs (7a, 7b), the two hindlimb positioning members (8a, 8b) being longer than the forelimb positioning members (6a, 6b); a trunk positioning member (10) which is longer than the hindlimb positioning members (8a, 8b), the trunk positioning member (10) being cylindrically attached to a trunk (9) of the animal (2) so as to wrap the trunk (9) between the forelimb positioning members (6a, 6b) which are attached to the forelimbs (5a, 5b) of the animal (2), respectively, and the hindlimb positioning members (8a, 8b) which are attached to the hindlimbs (7a, 7b) thereof, respectively; and a head positioning member (12) attached to a head (11) of the animal (2) so as to form a truncated cone circumferential surface and wrap the head (11) above the forelimb positioning members (6a, 6b).