Water borne diseases are estimated to have caused millions of deaths each year. These deaths are attributable to inadequate public sanitation systems. The main cause of these water borne diseases is the growth of micro-organisms in the water distribution system. These harmful microorganisms basically grow in the form of abiofilm. A biofilm is any group of microorganisms in which cells stick to each other on a surface. Biofilms may form on living or non-living surfaces and can be prevalent in natural, industrial and hospital settings. They not only affect human lives but also grow on the surfaces which can lead to corrosion in low pressure water boilers, reduce efficiency of heat exchanger surfaces, and reduce cooling tower efficiency. In order to inhibit the growth of biofilm in the drinking water distribution system, the Chlor-Z solution is synthesized and the experimental procedure is repeated to observe the effectiveness of the Chlor-Z solution on the E. coli growth on the entire pipe surfaces as well as on the surface of water tank used in the experimental setup. The Chlor-Z solution is divided into two solutions. First one is Chlor-Z-1 solution (3% Chloramine and 2 % Zinc Meta Phosphate) which is found to be very effective against the growth of biofilm in. pipe fittings in water distribution system as shown in the experimental set up. Second one is Chlor-Z-2 solution (8% Chloramine and 5% Zinc Meta Phosphate) which is highly effective against the microbial growth in open water tank system used for water distribution.